Overview:

• Inhibits SIRT1, supporting healthy expression of androgen receptor and activity.

• Supports healthy muscle growth in response to training.

Duration:

24 hours

Ingredients:

Dihydrocoumarin, bioactive flavours

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Application:

1ml daily with food in the morning

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Legal Status:

Legal in Australia, food additive FEMA

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Description:

Dihydrocoumarin has been shown to support healthy androgen receptor expression, and is a SIRT1 inhibitor pushing cells to differentiate and replicate. (1)

Polyphenols have anabolic and anti-androgenic effects and in some part act at associated proteins such as co-activators, co-repressors and estrogen receptors. Dihydrocoumarin is a naturally occurring (in this case nature identical) compound found in Sweet Clover (Melilotus officionalis). Research considered whether dihydrocoumarin could inhibit the human NADþ- dependent deacetylase SIRT1 and observed that DHC induced a concentration-dependent inhibition of SIRT1 (IC50 of 208 microM) in an in vitro enzymatic assay. That is loosely equivalent to 2400mg for an 80kg individual. A decrease in SIRT1 deacetylase activity was observed even at micromolar doses with observed activity at as low as 1.6 microM
(19mg). The microtubule SIRT2 deacetylase was also inhibited with a similar dose dependency. (6) SIRT1 is a repressor of anabolic and androgenic activity and inhibits DHT mediated transcription thus by inhibiting SIRT1 and SIRT2, healthy muscle growth in response to exercise is promoted. SIRT1 inhibition also supports healthy androgen receptor expression. (7)

 

1) Olaharski, A. J., Rine, J., Marshall, B. L., Babiarz, J., Zhang, L., Verdin, E., & Smith, M. T. (2005). The flavoring agent dihydrocoumarin reversesepigenetic silencing and inhibits sirtuin deacetylases. PLoS genetics, 1(6), e77.

2) National Toxicology Program. (1993). NTP Toxicology and Carcinogenesis Studies of 3, 4-Dihydrocoumarin (CAS No. 119-84-6) in F344/N Ratsand B6C3F1 Mice (Gavage Studies). National Toxicology Program technical report series, 423, 1.
3) Melis, M. S. (1999). Effects of chronic administration of Stevia rebaudiana on fertility in rats. Journal of ethnopharmacology, 67(2), 157-161.
4) Oliveira-Filho, R. M., Uehara, O. A., Minetti, C. A., & Valle, L. B. (1989). Chronic administration of aqueous extract of Stevia rebaudiana (Bert.)
Bertoni in rats: endocrine effects. General Pharmacology: The Vascular System, 20(2), 187-191.
5) Fang, H., Tong, W., Branham, W. S., Moland, C. L., Dial, S. L., Hong, H., ... & Sheehan, D. M. (2003). Study of 202 natural, synthetic, and
environmental chemicals for binding to the androgen receptor. Chemical research in toxicology, 16(10), 1338-1358.
6) Olaharski, A. J., Rine, J., Marshall, B. L., Babiarz, J., Zhang, L., Verdin, E., & Smith, M. T. (2005). The flavoring agent dihydrocoumarin reverses
epigenetic silencing and inhibits sirtuin deacetylases. PLoS genetics, 1(6), e77.
7) Fu, M., Liu, M., Sauve, A. A., Jiao, X., Zhang, X., Wu, X., ... & Pattabiraman, N. (2006). Hormonal control of androgen receptor function through
SIRT1. Molecular and cellular biology, 26(21), 8122-8135.